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300 nucleotide oligo pools opools  (Twist Bioscience)


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    Twist Bioscience 300 nucleotide oligo pools opools
    300 Nucleotide Oligo Pools Opools, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/300+nucleotide+oligo+pools+opools/Oligo+Pools/pmc12885220-53-8-15
    Average 99 stars, based on 1 article reviews
    300 nucleotide oligo pools opools - by Bioz Stars, 2026-09
    99/100 stars

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    Mutagenesis:

    Article Title: Functional and antigenic constraints on the Nipah virus fusion protein
    Article Snippet: .. To generate the F mutant libraries, we used 300 nucleotide oligo pools (oPools) synthesized by Twist Biosciences that contained all possible missense mutations and assembled them into lentiviral vectors using Golden Gate Assembly ( Fig. S2 ), an approach loosely based on previous work ( – ). .. We added barcodes consisting of 16 random nucleotides downstream from the stop codon of each F variant; these barcodes are subsequently linked to the full F sequence by long-read PacBio sequencing which then facilitates downstream sequencing by enabling each variant to be identified by Illumina sequencing of its barcode ( ).

    Article Title: Functional and antigenic constraints on the Nipah virus fusion protein
    Article Snippet: .. To generate the F mutant libraries, we used 300 nucleotide oligo pools (oPools) synthesized by Twist Biosciences that contained all possible amino-acid mutations to the ectodomain of F (site 29 to 481) and assembled them into lentiviral vectors using Golden Gate Assembly ( Fig. S3 ), an approach loosely based on previous work ( 44 – 46 ). .. We added barcodes consisting of 16 random nucleotides downstream from the stop codon of each F variant; these barcodes are subsequently linked to the full F sequence by long-read PacBio sequencing which then facilitates downstream sequencing by enabling each variant to be identified by Illumina sequencing of its barcode ( 40 ).

    Synthesized:

    Article Title: Functional and antigenic constraints on the Nipah virus fusion protein
    Article Snippet: .. To generate the F mutant libraries, we used 300 nucleotide oligo pools (oPools) synthesized by Twist Biosciences that contained all possible missense mutations and assembled them into lentiviral vectors using Golden Gate Assembly ( Fig. S2 ), an approach loosely based on previous work ( – ). .. We added barcodes consisting of 16 random nucleotides downstream from the stop codon of each F variant; these barcodes are subsequently linked to the full F sequence by long-read PacBio sequencing which then facilitates downstream sequencing by enabling each variant to be identified by Illumina sequencing of its barcode ( ).

    Article Title: Functional and antigenic constraints on the Nipah virus fusion protein
    Article Snippet: .. To generate the F mutant libraries, we used 300 nucleotide oligo pools (oPools) synthesized by Twist Biosciences that contained all possible amino-acid mutations to the ectodomain of F (site 29 to 481) and assembled them into lentiviral vectors using Golden Gate Assembly ( Fig. S3 ), an approach loosely based on previous work ( 44 – 46 ). .. We added barcodes consisting of 16 random nucleotides downstream from the stop codon of each F variant; these barcodes are subsequently linked to the full F sequence by long-read PacBio sequencing which then facilitates downstream sequencing by enabling each variant to be identified by Illumina sequencing of its barcode ( 40 ).



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    300 nucleotide oligo pools opools  (Twist Bioscience)
    99
    Twist Bioscience 300 nucleotide oligo pools opools
    300 Nucleotide Oligo Pools Opools, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/300+nucleotide+oligo+pools+opools/Oligo+Pools/pmc12885220-53-8-15
    Average 99 stars, based on 1 article reviews
    300 nucleotide oligo pools opools - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

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